Journal: Eukaryotic Cell

Article Title: MrSkn7 Controls Sporulation, Cell Wall Integrity, Autolysis, and Virulence in Metarhizium robertsii

doi: 10.1128/EC.00266-14

Figure Lengend Snippet: Cell wall integrity defect in the ΔMrSkn7 mutant. (A) The growth of the ΔMrSkn7 mutant (top row in each panel) was inhibited on PDA supplemented with 200 μg/ml calcofluor white (CFW) or 250 μg/ml Congo red (CR) for 2 days compared to the WT (bottom row in each panel). (B) Fluorescence staining of cell wall components with the lectin GSII (Griffonia simplicifolia) for chitin binding. (C) Staining with the lectin concanavalin A (ConA) to label the components of α-glucoside and α-mannose. (D) Staining with the lectin GNL (Galanthus nivalis lectin) to label α-mannose. Bars = 5 μm.

Article Snippet: These fluorescent lectins include the Alexa Fluor 488-labeled concanavalin A (ConA) (Invitrogen) (60 μg/ml) for binding α-mannopyranosyl and α-glucopyranosyl residues, lectin GSII (from Griffonia simplicifolia ) (Invitrogen) (20 μg/ml) for binding α- or β-linked N -acetyl- d -glucosamine (GlcNAc), and fluorescein-labeled Galanthus nivalis lectin (GNL) (Vector Laboratories) (20 μg/ml) for binding α-1,3-mannose ( 27 ).

Techniques: Mutagenesis, Fluorescence, Staining, Binding Assay

Journal: Immunity

Article Title: Vaccination Induces Maturation in a Mouse Model of Diverse Unmutated VRC01-Class Precursors to HIV-Neutralizing Antibodies with >50% Breadth

doi: 10.1016/j.immuni.2020.12.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The untagged Env immunogens, including eODGT6(8) 60mers, gp120 cores, and gp120 core ferritin nanoparticles, were purified with Galanthus nivalis (GNA)-lectin gel (EY Laboratories, Inc.) and followed with a size exclusion chromatography (SEC).

Techniques: Reverse Transcription, Staining, Sequencing, Expressing, Plasmid Preparation, Software

Journal: Immunity

Article Title: Vaccination Induces Maturation in a Mouse Model of Diverse Unmutated VRC01-Class Precursors to HIV-Neutralizing Antibodies with >50% Breadth

doi: 10.1016/j.immuni.2020.12.014

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The untagged Env immunogens, including eODGT6(8) 60mers, gp120 cores, and gp120 core ferritin nanoparticles, were purified with Galanthus nivalis (GNA)-lectin gel (EY Laboratories, Inc.) and followed with a size exclusion chromatography (SEC).

Techniques: Reverse Transcription, Staining, Sequencing, Expressing, Plasmid Preparation, Software

Journal: bioRxiv

Article Title: Biochemical characterization of the Escherichia coli surfaceome: A focus on type I fimbriae and flagella

doi: 10.1101/2024.01.14.575581

Figure Lengend Snippet: Protein and lectin staining of released E. coli surface proteins. NT: Non-treated, AH: Acid-hydrolyzed, LF: AIEC LF82. MG: E. coli MG1655. (A) Coomassie blue stain, (B) Anti-type I fimbriae-FITC staining, (C) ConA-HRP staining.

Article Snippet: HRP-conjugated galanthus nivalis lectin (GNA) was obtained from USBiological (CliniSciences, Nanterre; G1044-25).

Techniques: Staining

Journal: bioRxiv

Article Title: Biochemical characterization of the Escherichia coli surfaceome: A focus on type I fimbriae and flagella

doi: 10.1101/2024.01.14.575581

Figure Lengend Snippet: Lectin staining of released E. coli surface proteins. (A) GNA-HRP staining. Lane 1: Mock-treated LF82; Lane 2: PNGase F-treated LF82; Lane 3: Acid hydrolyzed E. coli MG1655; Lane 4: Untreated MG1655; Lane 5: Mock-treated MG1655; Lane 6: PNGase F-treated MG1655. C: PNGase F enzyme only control; M: Molecular weight ladder. (B) WGA-biotin staining. Lane 1: LF82 non-hydrolyzed; Lane 2: LF82 acid-hydrolyzed; Lane 3: MG1655 non-hydrolyzed; Lane 4: MG1655 acid-hydrolyzed.

Article Snippet: HRP-conjugated galanthus nivalis lectin (GNA) was obtained from USBiological (CliniSciences, Nanterre; G1044-25).

Techniques: Staining, Molecular Weight

Journal: bioRxiv

Article Title: Biochemical characterization of the Escherichia coli surfaceome: A focus on type I fimbriae and flagella

doi: 10.1101/2024.01.14.575581

Figure Lengend Snippet: ConA lectin probing following PNGase or mannosidase treatment of released E. coli surface proteins. LF: AIEC LF82, MG: E. coli MG1655. NT: Non-acid hydrolyzed, AH: Acid hydrolyzed, L: Molecular weight ladder, Mock: Mock-treated sample, Man: Mannosidase-treated sample. (A) PNGase F treatment probed with ConA. (B) Anti-type I fimbria staining of membrane (A). (C) Mannosidase treatment probed with ConA.

Article Snippet: HRP-conjugated galanthus nivalis lectin (GNA) was obtained from USBiological (CliniSciences, Nanterre; G1044-25).

Techniques: Molecular Weight, Staining, Membrane